Fig. 5

CCAT1 interacts with PTBP1. A, B The subcellular fractionation and FISH assays showed that CCAT1 was localized in both cytoplasm and nucleus of GC cells. C The catRAPID database indicated that PTBP1 had the most detected RNA-binding motifs of CCAT1. D The binding sites between PTBP1 and CCAT1. E The interaction between CCAT1 and PTBP1 was verified by RNA pull-down assay. F RIP assays confirmed the interaction between CCAT1 and PTBP1. G Colocalization of CCAT1 and PTBP1 in the nucleus of GC cells was captured under confocal laser scanning microscopy (Scale bars = 50 µm). H The GEPIA and I cBioPortal databases showed a positive correlation between the expression of CCAT1 and PTBP1 in GC tissues. J The expression of CCAT1 was positively associated with PTBP1 in 36 GC cases. The data are presented as the mean values ± SD from three independent experiments. Data were evaluated statistically with Unpaired t-test (F) and Spearman Correlations (J) using IBM SPSS statistics software version 21. ^**p < 0.01