Fig. 7

The role of PTBP1 in the regulation of glycolysis of GC cells. A The UALCAN database showed the expression of PTBP1 in GC and normal tissues. B The expression of PTBP1 was increased in 36 GC cases and was determined by IHC assays. C PTBP1 was elevated in GC cells compared with GES-1 at protein levels. D The positive correlations between PTBP1 and HK2, PKM2 and LDHA in GC tissues were verified by the cBioPortal database. E, F GC cells with PTBP1 knockdown or overexpression were constructed. G, H The expression of HK2, PKM2, LDHA, PKM1 and PKM1/PKM2 ratio was measured in GC cells with PTBP1 knockdown or overexpression. I ECAR was reduced in GC cells with PTBP1 knockdown, while that was elevated when PTBP1 overexpression. The data are presented as the mean values ± SD from three independent experiments. Data were analyzed by using Wilcoxon matched-pairs signed rank test (B) and Multiple unpaired t-tests (E and H). ^*p < 0.05, ^**p < 0.01