Fig. 8

CCAT1 promoted GC progression through PTBP1/glycolysis axis. A The repressed proliferation, B migration and C invasion of GC cells with CCAT1 knockdown were restored by PTBP1 overexpression. D Western-blot assays showed that the expression of PTBP1, HK2, PKM2 and LDHA was partially rescued in GC cells with CCAT1 knockdown and PTBP1 overexpression, while the expression of PKM1 had the opposite trend. E PTBP1 overexpression significantly rescued CCAT1 knockdown-induced inhibition of ECAR rates. The data are presented as the mean values ± SD from three independent experiments. Data were analyzed by 2-way ANOVA with Tukey's multiple comparisons test (A) and Ordinary one-way ANOVA with Tukey's multiple comparisons test (B and E) using GraphPad Prism 9 statistical software. ^*p < 0.05, ^**p < 0.01