Fig. 6

Knockdown of scaRNA2 increased sensitivity to chemoradiotherapy in cancer cells. A-D Survival of sg-ctrl and scaRNA2 knockdown HCT116 cells after irradiation (A), CPT (B), etoposide (C) or olaparib (D) treatment at the indicated doses (concentrations) was determined using a colony formation assay. **P < 0.01 compared with the sg-ctrl group at the same dose of treatment. E, F Cell apoptosis in sg-ctrl and scaRNA2 knockdown HCT116 cells after irradiation, CPT and ETO treatment was measured with flow cytometry using an Annexin V/PI double staining method. Quantification of Annexin V-positive cells is shown in the column graph. **P < 0.01, *P < 0.05 between the indicated two groups. G Proteins involved in the cell apoptosis signalling pathway were determined by western blotting in sg-ctrl and scaRNA2 HCT116 knockdown cells at the indicated times after 8 Gy irradiation. H Rectal cancer patient-derived organoids (PDOs) were cultured and infected with sg-ctrl and sg-scaRNA2 lentiviruses. Images were taken on Days 1–8 after irradiation at a magnification of 40X. The number of PDOs was calculated in each field from different groups. **P < 0.01 between the indicated two groups