Fig. 3

Generation and characterization of cell lines from BALB-neuT/xCT^wt and BALB-neuT/xCT^null tumors. A Fold change of the mean fluorescent intensity (MFI) of DCF signal or Oxydized Bodipy C11 signal 72 h after removal or not of β-ME from the growth medium, assessed through FACS analysis. B Percentage of dead (DAPI+) cells 72 h after removal of β-ME from the growth medium. C Proliferation curves, assessed by MTT assay, of SUT32-2H9 cells or WT27 cells in the presence or absence of β-ME. D Growth curves of 1 x 10⁶ SUT32-2H9 or WT27 cells injected subcutaneously in BALB/c mice. Number of replicates: each dot depicts a mouse (n = 5 per group in panel D) or an independent biological replicate. Statistical analysis: ratio paired t test (panel A) or unpaired t test (panels B and C). * p<0.05; ** p<0.01; *** p<0.001; Where not indicated, p value is not significant. Histograms (panels A, B) or lines (panel C) represent mean values. Error bars are not shown as n < 5