Fig. 3

Regnase-1 deletion in pancreatic tumor cells accelerates tumor progression with PMN-MDSC infiltration. A-E Evaluation of phenotypes of orthotopic syngeneic tumors of WT or Rengase-1 KO pancreatic cancer cells established from pancreas-specific Kras and Tp53 mutant (KPC) mice. Regnase-1 and Actb protein levels in murine pancreatic cancer cell lines (A) (Representative figure of triplicated experiments). Representative images of HE staining (B, top) and CD11b immunostaining (B, bottom). The number of CD11b-positive cells (B, right) (N = 6 per group) and tumor weights (C) (N = 14–16 per group). The relative mRNA levels of Itgam, Ly6g, Arg1, Nos2, S100a8, and S100a9 in the tumors (D) (N = 14–16 per group). Dot plots of CD45⁺CD11b⁺ cells (E, top) and CD45⁺CD11b⁺Gr-1 + cells (E, bottom) evaluated by flow cytometry. The proportion of CD11b + cells among CD45⁺ cells (E, right) (N = 6 per group). Student's t test was used to evaluate the differences between the two groups. *P < 0.05, scale bars: 100 μm (insets)