Fig. 3

Endogenous LBP has no significant effects on proliferation, apoptosis, invasion and migration, and mesenchymal transition of GC cells. A The effect of LBP on the proliferation in MKN45 cells was explored by EdU and colony formation assay with LBP stable overexpression or knockdown. Data are shown as mean ± standard deviation of 3 biologically independent experiments, however, the p values are not statistically significant. B Flow cytometry analysis of apoptosis was determined in MKN45 cells with stable LBP overexpression or knockdown. C The volumes of tumours were measured once a week after subcutaneous injection with MKN45 cells with stable LBP overexpression or knockdown. The growth curves of tumours are shown as mean ± SEM at sequential timepoints (n = 5 per group). D Photograph of subcutaneous tumours after mice were sacrificed in the 4th week. E Representative IHC images of Ki67 in subcutaneous tumours with LBP stable overexpression or knockdown. F Wound-healing assays (row 1, 2) and Transwell assays (row 3, 4) were applied to validate the effect of LBP on the migration and invasion of MKN45 cells. G Representative immunofluorescence images of EMT markers in MKN45 with LBP stable overexpression or knockdown. E-cadherin (red), Vimentin (green), and DAPI (blue). Scale bars are 100 μm (A, F), 50 μm (E), or 10 μm (G). Data are representative of three independent experiments. p values were determined by one-way ANOVA test (A) or two-way ANOVA test (C) (ns, not significant)