Fig. 5

GC-derived LBP are mainly accumulated in the liver and colocalized with intrahepatic macrophages. A Representative bioluminescent images of the liver, lung, bone, kidney and spleen tissues at the 24th hour after tail injection of reLBP-GFP (200 μg). B Representative IF images with partial amplification are shown that reLBP-GFP were mainly colocalized with macrophages (F4/80⁺ cells) in mouse liver after reLBP-GFP tail vein injection. C reLBP-GFP were mainly colocalized with macrophages (F4/80⁺ cells) in primary mixed liver cells in vitro. D Representative IF images shown that the CM from reLBP-pretreated THP-1 cells indues overexpression of fibrotic markers in HSC cell line (LX-2). E WB was applied to confirm that the CM from reLBP-pretreated THP-1 cells activated LX-2. F Representative H&E and IF images of F4/80, α-SMA, FN, and COL1 in the liver after pre-modelling with PBS, IgG, reLBP, reLBP combined with macrophage depletion, respectively. Data are representative of three independent experiments. Scale bars are 50 μm (B), 20 μm (C, D), 200 μm (F–H&E) and 100 μm (F-IF). Data are representative of three independent experiments