Fig. 6

Estrogen-related receptor alpha (ERRα) is involved in cell pyroptosis and participates in the reprogramming of energy metabolism based on TCGA data analysis. A Differential expression map of ERRα. ERRα expression in GTEx (78 cases) combined with TCGA paracancerous (23 cases) and TCGA endometrial cancer (181 cases) samples. Wilcoxon rank sum test was used for statistical analysis. B ERRα expression in normal (35 cases) and endometrial cancer (EC) tissues (552 cases) from TCGA. Wilcoxon rank sum test was used for statistical analysis. C ERRα expression in EC cancer tissues (23 cases) and the corresponding normal tissues from TCGA database. Paired sample t-test was used for statistical analysis. D ROC curves shows that ERRα (known as ESRRA) has high diagnostic accuracy in EC. E–F Associations between ERRα expression and residual tumor and histologic grade. G Using the Cox proportional hazard regression model, clinical stage, histologic grade, radiation therapy, primary treatment outcome, and residual tumor were included in the nomogram for predicting the probability of 1-, 3-, and 5-year OS in patients with EC. H Calibration plot of the nomogram for predicting the probability of OS in patients with EC at 1, 3, and 5 years. I GO analysis of TCGA data showed that ERRα-related genes in EC were enriched in glucose metabolism, pyruvate metabolism, programmed necrotic cell death, and glycolysis/gluconeogenesis. The subset of representative terms from this cluster was visualized with the p-value set to 0.05. J) Differential expression map of pyroptosis-related genes. The expression of pyroptosis-related genes in GTEx (78 cases) combined with TCGA paracancerous (23 cases) and TCGA endometrial cancer (181 cases) samples. Wilcoxon rank sum test was used for statistical analysis. K Pyroptosis-related gene expression in normal (35 cases) and EC tissues (552 cases) from TCGA. Wilcoxon rank sum test was used for statistical analysis. L Expression of pyroptosis-related genes in EC tissues (23 cases) and the corresponding normal tissues in TCGA database. Paired sample t-test was used for statistical analysis. M Analysis of GEO database data to show the correlation between the expression of ERRα and the pyroptosis pathway. N-P Survival analysis using ERRα,NLRP3, and GSDMD expression in EC. OS of patients with high versus low ERRα, NLRP3, and GSDMD expression levels. *p < 0.05; **p < 0.01; ***p < 0.001. Abbreviations: ERRα, estrogen-related receptor alpha; TCGA, The Cancer Genome Atlas; ROC, receiver operating characteristic; GO, Gene Ontology; OS, overall survival; ns, no statistical significance