Fig. 2

PGC-1α mediates LMP1-induced anoikis resistance in NPC cells. A CNE1/CM and HNE2/HM cells were cultured in suspension for 48 h, and then the transcription level of PGC-1α was detected by real-time fluorescence quantitative PCR. B CNE1/CM, HNE2/HM, CM-con/CM-shLMP1, and HM-con/HM-shLMP1 cells were cultured in suspension for 48 h, and the protein levels of PGC-1α were detected by Western blotting. C Survival rate of CNE1-con/CNE1-PGC-1α, HNE2-con/HNE2-PGC-1α, CM-con/CM-shPGC-1α, and HM-con/HM- shPGC-1α cells was assessed after 0, 2, 4, and 6 days suspension culture (**p < 0.01, *p < 0.05). D CNE1-con/CNE1-PGC-1α, HNE2-con/HNE2-PGC-1α, CM-con/CM-shPGC-1α, and HM-con/HM- shPGC-1α cells were cultured in suspension for 7 days, and the colony formation rate of the cells reattached was measured (**p < 0.01, *p < 0.05). E CNE1-con/CNE1-PGC-1α, HNE2-con/HNE2- PGC-1α, CM-con/CM-shPGC-1α, and HM-con/HM-shPGC-1α cells were cultured in suspension for 48 h and Western blotting was used to detect the expression of PGC-1α, cleaved-PARP-1 and cleaved-Caspase 3