Fig. 3

LMP1 promotes the PRMT1-PGC-1α interaction and induces arginine methylation of PGC-1α to enhance the stability of PGC-1α. A CNE1/CM and HNE2/HM cells were cultured in suspension and treated with CHX for 0, 1, 2, 4, 8, and 24 h, respectively, and the expression of PGC-1α protein was detected by Western blotting. B CNE1/CM and HNE2/HM cells were cultured in suspension for 48 h, and the arginine methylation level of PGC-1α was detected by Western blotting after purification of PGC-1α. C CNE1/CM and HEN2/HM cells were cultured in suspension, and the interaction between PRMT1-PGC-1α was detected by co- immunoprecipitation. D LMP1 was knocked down in CM and HM cells. Cells were cultured in suspension, and the interaction between PRMT1-PGC-1α was detected by co- immunoprecipitation. E PRMT1 was overexpressed in CNE1 and HNE2 cells, cultured in suspension for 48 h, and the arginine methylation level of PGC-1α was detected by Western blot analysis after purification of PGC-1α. F PRMT1 was overexpressed in CNE1 and HNE2 cells. The cells were cultured in suspension and treated with CHX for 0, 1, 2, 4, 8 and 24 h, respectively, and the expression of PGC-1α protein was detected by Western blotting. G The shRNA targeting PRMT1 was synthesized, and shRNA was transfected to CM and HM cells for 48 h, and then the expression level of PRMT1 was detected by Western blot analysis. H PRMT1 was knocked down in CM and HM cells. Cells were cultured in suspension for 48 h, and the arginine methylation level of PGC-1α was detected by Western blotting after purification of PGC-1α. I PRMT1 was knocked down in CM and HM cells. The cells were cultured in suspension and treated with CHX for 0, 1, 2, 4, 8 and 24 h, respectively, and the expression of PGC-1α protein was detected by Western blot analysis