Fig. 4

The C-terminal CTAR-2 domain of LMP1 promotes the interaction of PRMT1 with PGC-1α to enhance PGC-1α arginine methylation. A Structure diagram of each truncation of LMP1. B The LMP1 truncated plasmid, PRMT1 overexpression plasmid, and PGC-1α overexpression plasmid were simultaneously overexpressed in 293T cells, and the PRMT1-PGC-1α interaction was detected by co-immunoprecipitation. C GST-LMP1-WT, GST-LMP1-△C, GST-PRMT1, GST-PGC-1α-WT, and GST-PGC-1α-Mut fusion proteins were purified, and then visualized by Coomassie brilliant blue staining. D The GST-LMP1-WT and GST-PRMT1 proteins were incubated with GST-PGC-1α-WT or mutant protein GST-PGC-1α-Mut and AdoMet for in vitro methylation reaction. The level of arginine methylation modification of PGC-1α was detected by Western blotting. E GST-LMP1-WT, GST-LMP1-△C, and GST-LMP1-△CTAR-2 were incubated with GST-PRMT1 protein, GST-PGC-1α-WT protein, and AdoMet, respectively, and the level of arginine methylation modification of PGC-1α was detected by Western blot analysis