Fig. 5

PRMT1 mediates LMP1-inhibited PGC-1α ubiquitination by enhancing arginine methylation of PGC-1α. A PRMT1 was overexpressed in CNE1 and HNE2 cells. The cells were cultured in suspension and treated with the proteasome inhibitor MG132, and the expression of PGC-1α was detected by Western blotting. B PRMT1 was knocked down in CM and HM cells. The cells were cultured in suspension and treated with the proteasome inhibitor MG132. The expression of PGC-1α was detected by Western blotting. C PRMT1 was overexpressed in CNE1 and HNE2 cells. The cells were cultured in suspension and treated with the proteasome inhibitor MG132, and the ubiquitination level of PGC-1α was detected by in vivo ubiquitination assay. D PRMT1 was knocked down in CM and HM cells. The cells were cultured in suspension and treated with the proteasome inhibitor MG132, and the ubiquitination level of PGC-1α was detected by in vivo ubiquitination assay. E The FLAG-PGC-1α-WT/FLAG-PGC-1α-Mut and PRMT1 plasmids were transfected into 293T cells, and then 293T cells were treated with proteasome inhibitor MG132. The ubiquitination level of PGC-1α was then detected by in vivo ubiquitination assay. F The FLAG-PGC-1α-WT/FLAG-PGC-1α-Mut and shPRMT1 plasmids were transfected into 293T cells. The 293T cells were treated with the proteasome inhibitor MG132, and the ubiquitination level of PGC-1α was detected by in vivo ubiquitination assay