Fig. 7
PGC-1α mediates the invasion and metastasis of anoikis-resistant LMP1-positive NPC cells. A Flow chart of anoikis-resistant LMP1-positive NPC cell lines. B The survival rates of CM/CM-AR and HM/ HM-AR cells were detected after 0, 2, 4, and 6 days of suspension culture (**p < 0.01, *p < 0.05). C CM/CM-AR and HM/HM-AR cells were cultured in suspension for 7 days, and the invasive ability of the cells was detected by cell invasion assay (**p < 0.01, *p < 0.05). D CM-AR-con/CM-AR-sh PGC-1α cells and HM-AR-con/HM-AR-sh PGC-1α cells were cultured in suspension, and the survival rate of cells was detected at 0, 2, 4, and 6 days, respectively (**p < 0.01, *p < 0.05). E CM-AR-con/CM-AR-shPGC-1α cells and HM-AR-con/HM-AR-shPGC-1α cells were cultured in suspension for 48 h, and the protein expression levels of cleaved-PARP-1 and cleaved-Caspase 3 were detected by Western blotting. F CM-AR-con/CM-AR-sh PGC-1α cells and HM-AR-con/HM-AR-sh PGC-1α cells were cultured in suspension for 7 days, and the invasive ability of the cells was detected by a cell invasion assay (**p < 0.01, *p < 0.05). G Nude mice were subcutaneously injected with CM-AR-con and CM-AR-shPGC-1α cells, and the tumor volume and mass were detected at 14 days (**p < 0.01, *p < 0.05). H Immunohistochemistry was used to analyze the expression of PGC-1α, PD-L1, cleaved-Caspase 3, and Ki67 in mouse tissue sections (**p < 0.01, *p < 0.05). I Nude mice were subcutaneously injected with CM-AR-con and CM-AR-shPGC-1α cells, and the infiltration of the tumor margins was detected by H&E staining. J Nude mice were subcutaneously injected with CM-AR-con and CM-AR-shPRMT1 cells, and the tumor volume and mass were detected at 14 days (**p < 0.01, *p < 0.05). K Immunohistochemistry was used to analyze the expression of PRMT1, PGC-1α, and Ki67 in mouse tissue sections (**p < 0.01, *p < 0.05). L Flow chart of lung metastasis of anoikis-resistant LMP1-positive NPC cells in nude mice. M CM-AR-con and CM-AR-shPGC-1α cells were injected into the tail vein of nude mice, and 48 days later, the lung metastatic nodules were observed by H&E staining (**p < 0.01, *p < 0.05)