Fig. 2

Chronic stress activates parasympathetic nervous system and promotes acetylcholine secretion in lungs. a-b. Immunofluorescence examinations of TH (a) and VAChT (b) expression in lung tissues from breast cancer model mice with or without chronic restraint stress for 2 weeks. c. Immunofluorescence examination of VAChT expression in lung tissues from breast cancer model mice with or without unpredictable stress for 2 weeks. d. qRT-PCR (top) and Western blot analyses of ChAT (middle and bottom) in lung tissues from breast cancer model mice with or without chronic restraint stress for 2 weeks. e. qRT-PCR (left) and Western blot analyses (middle and right) of VAChT in lung tissues of mice in control and chronic restraint stress groups at 2 weeks. f. ACh concentrations in serum and lung cultural supernatant of breast cancer model mice in control group and chronic restraint stress group for 2 weeks. ns: no sense, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001