Fig. 4

Chronic stress induces infiltration of neutrophils into lung. a. Gating strategy of flow cytometry analyses of immune cell subsets. b. FACS analyses of immune cell subsets in lungs from orthotopic inoculation murine breast cancer model with or without restraint stress. c. FACS analyses of Ly6G^hiCD11b⁺ neutrophils in lungs from murine breast cancer model with or without chronic restraint stress for 2 weeks. d. Percentages of Ly6G^hi/CD11b⁺ neutrophils in lungs from breast cancer model mice with or without chronic unpredictable stress. e. FACS analyses of neutrophils in lungs from model mice with or without chronic unpredictable stress for 2 weeks. f. FACS analyses of neutrophils in lungs from 14-week-old PyMT-MMTV spontaneous breast cancer model mice with or without chronic restraint stress for 6 weeks (upper) and their corresponding statistical results (bottom). ns: no sense, **p < 0.01, ***p < 0.001, ****p < 0.0001