Fig. 5

Chronic stress induces the chemotaxis of neutrophils into lung via CXCL2-CXCR2 axis. a. Transwell chemotaxis assay to detect the chemotaxis activity of lung culture supernatants to peripheral blood neutrophils of model mice in the control group at 1 week. CLCS: lung culture supernatant of model mice in control group; SLCS: lung culture supernatant of model mice in chronic restraint stress group at 2 weeks. b. Transwell chemotaxis assay to detect the chemotaxis activity of ACh to peripheral blood neutrophils from model mice in the control group at 1 week. c. Chemokine array to determine the contents of different chemokines in lung culture supernatants from orthotopic inoculation breast cancer mice with or without chronic restraint stress for 2 weeks. d. Transwell chemotaxis assay to examine the influence of CXCL2 neutralizing antibody on neutrophil chemotactic activity of SLCS. DMEM: culture medium to serve as the blank control; CLCS: lung culture supernatant of mice in control group; SLCS: lung culture supernatant of mice in chronic restraint stress group at 2 weeks; SLCS + iCXCL2: SLCS + 1 μg/ml CXCL2 neutralizing antibody; CXCL2: 2ng/ml. e. Cell counts of experiments as described in d. f. qRT-PCR to dynamically detect the mRNA levels of CXCR2 in lung neutrophils from breast cancer model mice with or without chronic restraint stress. ns: no sense, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001