Fig. 6

Chronic stress enhances NETosis of neutrophils in the lung of model mice. a. Immunofluorescence analyses of in situ NETosis of neutrophils in lung tissues of breast cancer model mice with or without chronic restraint stress for 2 weeks. b. NETosis percentages of neutrophils in experiments as described in a. c. Immunofluorescence to examine NETs structures of neutrophils isolated from lungs of model mice in control group and in chronic restraint stress group at 2 weeks. d. NETosis percentages of neutrophils in experiments as described in c. e-f. qRT-PCR and Western blot to detect mRNA (e) and protein (f) levels of PADI4 in neutrophils isolated from lungs of model mice in control group and in chronic restraint stress group at 2 weeks. **p < 0.01, ***p < 0.001