Fig. 9From: Chronic stress induces pulmonary epithelial cells to produce acetylcholine that remodels lung pre-metastatic niche of breast cancer by enhancing NETosisImmunohistochemistry analyses of ChAT expression in lung tissues of breast cancer patients with lung metastasis. a. Representative immunohistochemistry images of ChAT expression in lung tissues from patients with non-tumor bearing pulmonary diseases and from breast cancer patients with lung metastasis. b. Average optical density (upper) and percentage of ChAT positive area to total area (bottom) analyzed by ImageJ software. **p < 0.01Back to article page