Fig. 1
NMRK2 promoted mitochondrial respiration and tumor growth of NONO-TFE3 rRCC. A UOK109 cells were transfected with lentivirus shRNA (NC) or shRNA (NMRK2). The mitochondrial respiration of cells in each group was measured by the Seahorse XF Extracellular Flux Analyzer with a Seahorse XF Cell Mito Stress Kit, B and analyzed with GraphPad Prism 8. C And the cell proliferation viability was analyzed with a CCK-8 assay kit. D UOK109 cells were transfected with lentivirus shRNA (NC) or shRNA (NMRK2) and cultured in a medium with glucose or galactose. The ATP production was detected with an ATP assay kit. E HK-2 cells were transfected with lentivirus pCDH-DsRed/pCDH-NT, shRNA (NC)/shRNA (NMRK2). Twenty female nude mice were randomly divided into 4 groups with 5 mice per group. Then 1 × 106 transfected HK-2 cells were injected subcutaneously in mice. After 4 weeks, the tumors from each group were removed and measured. F The expression of Ki67 and TFE3 protein of the tumors from each group was detected by IHC. G The statistic results of Ki67 and TFE3 were calculated with Image J and GraphPad Prism 8. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001