Fig. 7

LncRNA like NMRK2 mRNA promoted the mitochondrial respiration of NONO-TFE3 rRCC by upregulating the protein stability of Hsp10. A UOK109 cells were co-transfected with dCas13b-HA and sg-Control/sg-NMRK2, and the NMRK2 binding protein was detected by IP-MS assay. B UOK109 cells were transfected with lentivirus shRNA (NC) or shRNA (HSPE1), and the expression of NMRK2 was detected by q-PCR. 18S rRNA was used as the reference gene. C UOK109 cells were transfected with lentivirus shRNA (NC) or shRNA (HSPE1) and treated with 10 μM ACTD for 0, 6, 12, 24, and 30 h. The expression of NMRK2 at various time points was detected by q-PCR. 18S rRNA was used as the reference gene. D UOK109 cells were transfected with lentivirus shRNA (NC) or shRNA (NMRK2), and the expression of the Hsp10 protein was detected by Western Blotting. β-actin was used as the internal reference. E UOK109 cells were transfected with lentivirus shRNA (NC) or shRNA (NMRK2) and treated with 40 μM CHX for 0, 2, 4, 6, 12, and 24 h. The expression of Hsp10 protein at various time points was detected by WB. β-actin was used as the internal reference. F, G UOK109 cells were transfected with lentivirus shRNA (NC), shRNA (NMRK2), or shRNA (HSPE1), and the apoptosis was assessed by Flow cytometry analysis and Western Blotting. β-actin was used as the internal reference. H The ATP production and I mitochondrial membrane potential of cells in each group was detected with an Enhanced ATP Assay Kit and a Mitochondrial Membrane Potential Assay Kit. Data are presented as the mean ± SEM. **P < 0.01, ***P < 0.001