Fig. 4

CSRP2BP promotes cervical cancer metastasis in vitro and in vivo. A Wound healing assays indicated that the migration rate increased in CSRP2BP-overexpressing cells and decreased in CSRP2BP knockdown cells. The imgration rates are expressed as the area percentage at 0 h (scale bar 100 µm). Data are shown as the mean ± SD (n = 6). B Transwell assays showed that the invasion rates increased in CSRP2BP-overexpressing cells and decreased in CSRP2BP knockdown cells (scale bar 20 µm). Data are shown as the mean ± SD (n = 3). C Hela-CSRP2BP, Hela-shCSRP2BP and the respective control cells were injected into BALB/c-nude mice through the tail vein (n = 5). Representative photos of lungs isolated from mice. Green fluorescence was derived from EGFP of cells using a chemiluminescent imaging system in vitro imaging. D H&E staining of representative samples derived from mice lungs (scale bars, 40 µm and 20 µm, respectively). E, F Lung weight and the number of metastatic nodules were significantly increased in Hela-CSRP2BP cells and decreased in Hela-shCSRP2BP cells. Data are shown as the mean ± SD. (*P < 0.05, **P < 0.01, ***P < 0.001)