Fig. 5

CSRP2BP activates N-cadherin expression to enhance cervical cancer cell metastasis. A Heatmap representation of the top-ranked genes upregulated and downregulated in Hela-CSRP2BP cells compared with the control group. B The GSEA plot revealed that CSRP2BP expression levels were positively correlated with EMT. C The protein expression levels of N-cadherin and E-cadherin in Hela-CSRP2BP and Hela-shCSRP2BP cells were detected by Western blotting. D The trend in the expression of CSRP2BP, N-cadherin and E-cadherin by IHC is consistent in metastatic tumors of mice lungs. (Scale bar 20 µm). E Hela-CSRP2BP cells and control cells were treated with siRNA targeting N-cadherin for 48 h. N-cadherin silencing in Hela-CSRP2BP cells was confirmed by Western blotting. F Wound healing and Transwell assays G indicated that the migration and invasion rates decreased in Hela-CSRP2BP-siN-cadherin cells. H Hela-shCSRP2BP and control cells were transfected with pSin-N-cadherin and control plasmids. N-cadherin overexpression in Hela-shCSRP2BP cells was confirmed by Western blotting. I Wound healing and Transwell assays J indicated that the migration and invasion rates increased in Hela-shCSRP2BP-N-cadherin cells. Data are shown as the mean ± SD (n = 6). Data are shown as the mean ± SD (n = 6). (*P<0.05, **P < 0.01, ***P < 0.001)