Fig. 3

Hypoxia-induced SKA3 promoted CCA progression by enhancing fatty acid synthesis. A KEGG pathway analysis of downregulated genes in SKA3-KD HuCCT1 cells. B GO analysis of downregulated genes in SKA3-KD HuCCT1 cells. C Validation of lipogenic enzyme genes through FPKM normalized from three independent samples. D RT-qPCR analysis of mRNA expression of lipogenic enzyme genes FASN, ACLY, ACACA, and SCD in HuCCT1 and QBC939. E Protein levels of lipogenic enzymes were determined by western blotting in HuCCT1 and QBC939 cells. F Cellular neutral lipids were measured in HuCCT1 and QBC939 cells by double staining with Nile Red and DAPI. G Cellular triglycerides were measured in HuCCT1 and QBC939 cells, which normalized by NC group. H The ATP concentration in HuCCT1 and QBC939 cells were tested by ATP Assay Kit. I Oil red staining was performed in SKA3 highly expressed tumour tissues and paired para-tissues. J Scatter plot analysis of correlation between mRNA levels of SKA3 and FASN, ACLY, ACACA, or SCD in 70 CCA tissues. *P < 0.05, **P < 0.01, ***P < 0.001