Fig. 8

Hypoxia-induced SKA3 protects CCA cells against the cytotoxic effects of gemcitabine. A CCA cells with SKA3 overexpression were treated with gemcitabine at different dose under hypoxic conditions, then cell viability was examined after 48 h treatment. C CCA cells with SKA3 overexpression group and NC group were treated with gemcitabine at dose of 500 nM/mL under hypoxic conditions, then clone formation assays (B) and DNA damage detection assays (C) were performed. D-F The efficacy of gemcitabine in vivo was determined in nude mice injected with SKA3 overexpression QBC939 cells. G Representative images of H&E staining in tumour derived from DMSO or gemcitabine-treated nude mice with SKA3 overexpression group and NC group cells inoculation (scale bar: 50μm). *P < 0.05