Fig. 3

Combination therapy of TMZ and ABX potentiated DNA damage and impeded DNA damage repair in GBM cells. a Protein levels of γ-H2AX were analyzed by western blotting in GBM cells treated with TMZ (400 µM for U87-MG and G353, 800 µM for LN229 and G393) with or without ABX (12 nM) for 48 h. b-d Typical comet assay images of GBM cells treated with the indicated drugs for 48 h in each group (b), as well as quantification and statistical analysis of DNA in the tail (c) and tail moment (d). e Schematic illustration of dynamic detection of γ-H2AX foci at various time points after drug treatment is indicated to monitor the course of DNA damage repair. f Left, dynamic changes of γ-H2AX foci were observed in GBM cells treated with TMZ with or without ABX at various time points following drug elution. Right, statistical analysis was conducted on the number of γ-H2AX foci in GBM cells at various time points following drug elution. Scale bar, 10 µm