Fig. 1

METTL16 is upregulated in CCA and correlates with poor prognosis. A, B. METTL16 expression in CCA tissues versus matched (left) or nonmatched (right) non-tumorous tissues from TCGA (A) and GSE107943 (B) datasets. C. METTL16 expression in 10 primary and 7 advanced CCA tumors in the GSE107101 dataset. D. Kaplan–Meier survival plot of CCA patients from the TCGA database stratified by low (red) and high (blue) METTL16 expression. E. Representative immunochemistry (IHC) staining images of METTL16 expression in the normal bile duct and CCA tissues. Scale bar: 10 µm. F. Quantitative result of IHC data as represented in E. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. A-C, E Unpaired t-test