Fig. 2

Depletion of METTL16 inhibits CCA cell proliferation and reduces clonogenicity, in vitro. Human CCA cells (HuCCT1 and CCLP1) were transfected with siRNAs targeting METTL-16 (siMETTL16-1 and siMETTL16-2) and control siRNA (siNC) or transfected with CRISPR/Cas-9 vector carrying METTL16-specific guide RNAs (sgMETTL16-1 or sgMETTL16-2). A Western blot for METTL16 in CCA cells transfected with control (siNC) or METTL16 targeting siRNAs. B WST-1 cell proliferation assay in CCA cells transfected with control (siNC) or METTL16 targeting siRNAs. C Colony formation assay in CCA cells transfected with control (siNC) or METTL16 targeting siRNAs. D Western blot for METTL16 in CCA cells with or without CRISPR/Cas9-mediated METTL16 deletion. E WST-1 cell proliferation in CCA cells with or without CRISPR/Cas9-mediated METTL16 deletion. F Colony formation assay in CCA cells with or without CRISPR/Cas9-mediated METTL16 deletion. **P < 0.01, ***P < 0.001, ****P < 0.0001. B, C, E, F Mean ± SD, One-way ANOVA test