Fig. 5

PRDM15 is essential for METTL16-mediated CCA cell growth. A-C Depletion of PRDM15 inhibits CCA cell proliferation and colony formation capability. The efficacy of PRDM15 depletion by siRNA was validated by immunoblotting analysis (A). WST-1 cell proliferation (B) and colony formation (C) assay were performed in CCLP1 and HuCCT1 cells transfected with siPRDM15 and control siRNA. D-F Forced overexpression of PRDM15 rescues the deficiency of CCA cell proliferation and colony formation induced by METTL16 depletion. PRDM15 expression construct was transfected in CCLP1 and HuCCT1 cells for 48 h and PRDM15 protein expression was determined by immunoblotting (D). WST-1 cell proliferation (E) and colony formation (F) assay were performed in CCLP1 and HuCCT1 cells transfected with PRDM15 overexpression or control construct. **P < 0.01, ***P < 0.001, ****P < 0.0001. B, C, E, F Mean ± SD, One-way ANOVA test