Fig. 7

Regulation of METTL16 expression by p300-mediated H3K27ac in CCA. A Analysis of the UCSC Genome Browser (http://genome.ucsc.edu/) indicates that the promoter region of METTL16 is highly enriched with H3K27ac. B ChIP-qPCR assay using anti-H3K27ac revealed a gain of H3K27ac at the METTL16 promoter in CCLP1 cells. C ChIP-qPCR assay using anti-p300 and anti-CBP showed enrichment of p300 at the METTL16 promoter in CCLP1 cells. D, E Enrichment of H3K27ac (D) and p300 (E) at the promoter region of METTL16 was evaluated by ChIP-qPCR assay in CCLP1 cells treated with C646 (20 µM) for 48 h. F METTL16 mRNA expression level was determined by RT-qPCR in CCLP1 and HuCCT1 cells treated with C646 (20 µM) for 48 h. G METTL16 protein expression level was analyzed by immunoblotting in CCLP1 and HuCCT1 cells treated with C646 at indicated time points. ***P < 0.001, ****P < 0.0001. (B) Mean ± SD, Unpaired t-test. C-F Mean ± SD, Two-way ANOVA test