Fig. 8

p300 cooperates with YY1 to regulate METTL16 expression. A Venn diagram displays overlapping genes of p300 interactors (BioGrid) and predicted transcription factors (TFs) for METTL16 (SwissRegulon). B Spearman correlation analysis of METTL16 and the identified TFs in CCA tissues from the TCGA database. C ZBTB3, TBLXR1, YY1, SP1, and SP3 expression in CCA and normal tissues from the TCGA database. D Kaplan–Meier survival plot of CCA patients from TCGA database stratified by low (blue) and high (red) YY1 expression. E The interaction between p300 and YY1 in CCLP1 and HuCCT1 cells was validated by immunoprecipitation assay. F The binding sites of YY1 on the promoter region of METTL16 were predicted by JASPAR (top). ChIP-qPCR was performed in CCLP1 and HuCCT1 cells treated with C646 (20 µM) for 48 h (bottom). G Proposed model for the roles of METTL16 in CCA. (Created using tools from BioRender) *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. C, F Mean ± SD, Two-way ANOVA test