Fig. 1

FTO-IT1 was upregulated in HCC samples and might function as a tumor-promoting lncRNA. A Venn diagram indicating the identification of differentially expressed lncRNAs among GSE98269, GSE101728 and TCGA datasets. B Log-rank test of OS and DFS of the 7 lncRNAs which screening from the aforementioned three datasets. C The relative expression of the 7 lncRNAs were detected in 92 HCC clinical specimens by qRT-PCR. D Kaplan–Meier survival analysis using online tool GEPIA (http://gepia.cancer-pku.cn) showed the OS and DFS of FTO-IT1. E Gene set enrichment analysis of FTO-IT1-correlated genes in TCGA database. NES, normalized enrichment score. F qRT-PCR detected the subcellular fractions of FTO-IT1 in HepG2 and MHCC97H cells (left). The qRT-PCR products were separated by 2% agarose gel electrophoresis; U6 and β-actin were used as markers of the nucleus and cytoplasm, respectively (right). G RNA-FISH analysis of FTO-IT1 (red) in HepG2 and MHCC97H cells. Scale bars, 20 μm