Fig. 2

FTO-IT1 promoted glycolysis and proliferation of HCC cells. A qRT-PCR showed the relative levels of FTO-IT1 in normal liver cell line (MIHA) and HCC cell lines. B Knockdown efficiency of two siRNAs of FTO-IT1 was assessed via transfection assay followed by qRT-PCR. C-E Colony formation assays (C), EdU assays (D) and MTT assays (E) were used to evaluate the proliferation ability of HepG2/MHCC97H cells transfected with siFTO-IT1 or siNC. F The glucose uptake (left), lactate production (middle) and pH value (right) of HCC cells transfected with siNC or siFTO-IT1