Fig. 3

FTO was a critical target for FTO-IT1 regulating glycolysis. A Schematic illustration showing the genomic location of FTO-IT1 and FTO. B The expression correlation between FTO-IT1 and FTO in HCC tissues derived from the TCGA database. C The expression of FTO in HCC tissues and normal liver tissues by using the online database UALCAN (http://ualcan.path.uab.edu/analysis.html). NT: non-tumor. D The expression of FTO in different stages of HCC by using the online database UALCAN. E Gene set enrichment analysis of FTO-correlated genes in TCGA database. NES, normalized enrichment score. FDR, false discovery rate. F The mRNA (left) and protein (right) level of FTO after knocking down FTO-IT1. G The glucose uptake (left), lactate production (middle) and pH value (right) of HCC cells transfected with siNC or siFTO. H-J The proliferation of HepG2/MHCC97H cells transfected with siNC, siFTO-IT1 and empty vector or overexpression FTO plasmid and cultured with or without 2DG (5 mmol/L) was evaluated by colony formation assays (H), EdU assays (I) and MTT assays (J)