Fig. 5

FTO facilitated glycolysis in HCC cells by targeting glycolytic enzymes. A Venn diagram indicating the identification of glycolysis-related genes were differentially expressed (fold change > 2.0, P < 0.05) in TCGA LIHC datasets with clinical stages, prognosis of survival and correlation with FTO (R > 0, P < 0.05). B, C The mRNA (B) and protein (C) expression of glycolysis-related genes in HepG2/MHCC97H cells transfected with siNC, siFTO or in Huh7 cells transfected with empty vector, overexpression FTO plasmid. D, E The correlation of the expression between FTO and GLUT1 (D) or PKM2 (E) (left). The relative transcript levels of GLUT1 (D) or PKM2 (E) in HCC tissues with different pathological stages (middle). Kaplan–Meier analyses of OS in HCC patients with low and high levels of GLUT1 (D) or PKM2 (E) (right) from GEPIA online database