Fig. 6

FTO-IT1/FTO signaling increased mRNA stabilization of GLUT1 and PKM2 by decreasing m⁶A modification. A Pie charts analysis showing the m⁶A modification sites of all sources of GLUT1 and PKM2 from the RMvar online database (https://rmvar.renlab.org/index.html). B Global m⁶A levels in mRNA were detected using colorimetric method in HepG2/MHCC97H cells transfected with siNC, siFTO (left) or in Huh7 cells transfected with empty vector, overexpression FTO plasmid (right). C MeRIP assays were applied using the anti-m⁶A antibody and IgG antibody in HepG2 cells. qRT-PCR was used to detect the corresponding enrichment of GLUT1 and PKM2. D, E MeRIP assays were applied using the anti-m⁶A antibody and IgG antibody in HepG2 cells transfected with siNC, siFTO (D) or in Huh7 cells transfected with empty vector, overexpression FTO plasmid (E) to detect the corresponding enrichment of GLUT1 and PKM2. F, G Cells transfected with siNC, siFTO-IT1 (F), or empty vector, overexpression FTO plasmid (G), and treated with ActD for corresponding hours followed by qRT-PCR assays for GLUT1 and PKM2 mRNA. H The mRNA (left) and protein (right) levels of GLUT1 and PKM2 in HepG2 cells transfected with siFTO-IT1 and/or overexpression FTO plasmid. I The mRNA (left) and protein (right) levels of GLUT1 and PKM2 in Huh7 cells transfected with overexpression FTO-IT1 plasmid and/or siFTO