Fig. 3

PRV specifically triggers severe ER stress to induce apoptosis in cancer cells. A Observation of ER swelling/distension in Panc-1 cells infected with PRV-LAV HB2000 by TEM. High-magnification images are also presented. Scale bars, 500 nm. B Quantification of ER distension in (A). Data are presented as the mean ± s.d. values (n = 40). Statistical analysis was performed by one-way ANOVA. C-G Effect of PRV-LAV on the ER stress-induced apoptosis signaling. Western blot analysis of Bip (C), phosphorylated eIF-2α and eIF-2α (D), phosphorylated JNK and JNK (E), caspase-12 (F), cleaved caspase-3, cleaved PARP, and PRV gB (G). GAPDH was used as a loading control. H-I Caspase-9 (H) and caspase-3/7 (I) activity in Panc-1, HepG2, and HPNE cells treated with PRV-LAV HB2000 (MOI = 1). Data are presented as the mean ± s.d. values (n = 3). TEM, transmission electron microscopy