Fig. 3

Mitochondrial gene expressions were decreased in dormant C4-2B cells.(A). Workflow of RNA-sequencing analyses. (B). Gene Ontology (GO) annotation of biological process in dormant C4-2B cells via ShinyGO v0.61. (C). qRT-PCR confirmation of representative mitochondrial gene expression changes in the co-cultured C4-2B cells relative to the cells cultured alone. (D). Analyses of mitochondrial DNA (mtDNA) copy numbers via qRT-PCR. C4-2B cells were cultured alone or co-cultured with MC3T3 cells for 72 h. All the experiments except for the RNA-Seq were independently repeated at least three times. The data of bar plots are presented as the mean ± SD of each set of triplicate samples. ** p < 0.01, *** p < 0.001