Fig. 6

PF-271 mimicked C4-2B dormancy by blocking FAK phosphorylation and promoting FAK nuclear translocation. (A). C4-2B/GFP-FAK cells were treated with 1 µM PF or Veh (Vehicle, DMSO) for 24 or 72 h. The protein levels of pY397-FAK, total FAK, and CCND1 (cyclin D1) were examined by immunoblotting. (B). C4-2B/GFP-FAK cells were treated with 1 µM PF or Veh (Vehicle, DMSO) for 24 h and monitored every 2 h on IncuCyte S3. Representative pictures at 24 h are shown. (C). C4-2B cells were co-cultured with MC3T3-E1 or cultured alone for 72 h. The protein levels of total FAK and NR2F1 were examined by immunoblotting (left). Phospho-FAK levels were examined with immunostaining and captured with confocal microscopy. Red, pY397-FAK; blue, DAPI, nucleus. (D). C4-2B/GFP-FAK cells were co-cultured with MC3T3-E1 or cultured alone for 72 h. Representative pictures at 72 h are shown. Scale bars, 50 μm. All the experiments were independently repeated at least three times