Fig. 4

TOP2A is a direct target of miR-139-5p. (A) Overlap of miRNAs differentially expressed in hepatocellular carcinoma and adjacent normal tissue in the GSE185913 set and miRNAs targeting TOP2A in the TargetScan and miRcode databases. (B) Correlation analysis of miR-139-5p and TOP2A in HCC using starBase v2.0. (C) Correlation analysis of miR-139-5p and TOP2A in HCC cell lines. (D) Putative binding site of miR-139-5p in the TOP2A 3’UTR and dual luciferase reporter assay of miR-139-5p and TOP2A 3’UTR in HEK293T, BEL7404 and SMMC7721 cell lines. (E) RT‒qPCR of miR-139-5p in BEL7404 and SMMC7721 cells transfected with miR-139-5p mimics. (F) Western blot of TOP2A in BEL7404 and SMMC7721 cells transfected with miR-139-5p mimics. (G) RT‒qPCR of miR-139-5p in BEL7404 and SMMC7721 cells transfected with miR-139-5p inhibitor. (H) Western blot of TOP2A in BEL7404 and SMMC7721 cells transfected with miR-139-5p inhibitor. *p < 0.05, **p < 0.01, ***p < 0.001