Fig. 6

miR-139-5p inhibits cell proliferation and induces senescence in HCC. (A) Representative images of colony formation and SA-β-gal staining of cells stably expressing plenti6.3 and plenti6.3-miR-139. (B) mRNA levels of miR-139-5p and senescence markers p15, p16, and p21 in HCC cells overexpressing miR-139. (C) Representative images of tumors in nude mice subcutaneously inoculated with BEL7404 cells stably expressing plenti6.3 and plenti6.3-miR-139 (seven mice in each group). (D and E) The tumor volume (D) and weight (E) in nude mice subcutaneously inoculated with BEL7404 cells stably expressing plenti6.3 and plenti6.3-miR-139. (F) Representative images of H&E, Ki67, TOP2A, and SA-β-gal staining of tumor tissues. (G) The cell rate of Ki67-positive cells in tumors. (H) The cell rate of TOP2A-positive cells in tumors. (I) The cell rate of SA-β-gal-positive cells in tumors. (J) mRNA levels of miR-139-5p, TOP2A, and senescence markers p15, p16, and p21 in tumors in nude mice subcutaneously inoculated with BEL7404 cells. *p < 0.05, **p < 0.01, ***p < 0.001