Fig. 1

Preparation of washed platelets and establishment of the TCIPA metastatic melanoma model (B16 + PLT model). a Scheme for the preparation of washed platelets. WBC, white blood cell; RBC, red blood cell; centrifuge 1: 156 × g for 7 min, centrifuge 2: 300 × g for 7 min, centrifuge 3: 1000 × g for 4 min, all with no brake. b The human-derived and (c) mouse-derived washed platelets were observed under 100 × , 200 × , and 400 × magnification. d Flowchart for establishment of the metastatic melanoma model (B16 model) and the TCIPA metastatic melanoma model (B16 + PLT model). Photographs and H&E staining images of (e) lung metastases and (f) primary tumors in the B16 + PLT model, B16 model and control groups. g-h When compared with the B16 model, the B16 + PLT model produced a significantly greater number of lung metastases (N = 8) and heavier lung weight (N = 9). i-j For the primary tumor weight and volumes (N = 6), there was no significant difference between the two models. *p ≤ 0.05, ***p ≤ 0.001