Fig. 2

TCIPA in vivo is enhanced in the B16 + PLT model but does not directly promote the migratory capacity of B16 cells. The expression of (a) CD42b and (b) CD62p in the lung metastases was observed by IHC assay under 100 × , 200 × , and 400 × magnification. Chocolate brown: positive expression; calamine blue: nucleus. As shown in graphs (d) and (e), the integrated optical density (IOD) values of CD42b and CD62p were significantly different between the B16 + PLT and B16 models. **p ≤ 0.01, ***p ≤ 0.001, N = 15. c Modified MSB staining images. Incarnadine: fresh fibrin; bluish violet: stale fibrin; bluish-brown: nucleus; yellow: RBCs; sapphire: collagen fibers; gray: platelet trabeculae. f Peripheral platelet count. *p ≤ 0.05, N = 5. g-i Platelet-stimulated B16 cells showed neither faster wound healing (***p ≤ 0.001, N = 3) nor greater migratory capacity than B16 cells alone (no significant difference, N = 5)