Fig. 3

TCIPA alters the phenotype and number of macrophages in the TIME. a The FCM protocol. b The proportion of F4/80⁺ CD11b⁺ CD206⁺ cells among the live CD45⁺ cells in the fresh spleens (e), lung metastases (f) and primary tumors (g) from the B16 + PLT model, B16 model and control groups. *p ≤ 0.05, **p ≤ 0.01, N = 3. The expression of F4/80 (c) and CD206 (d) in lung metastases was detected by IHC. The IOD values of both F4/80 (h) and CD206 (i) were significantly higher in the B16 + PLT model than in the B16 model. ***p ≤ 0.001, N = 15