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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Autocrine regulation of tumor cell repopulation by Hsp70-HMGB1 alarmin complex

Fig. 2

Hsp70 resencein CM increases the repopulation of tumor cells. (A) Western blotting of A549 and DLD1 cells with normal and reduced Hsp70 levels treated with 100µM oxaliplatin during 12 h. (B) Western blot of CL of A549 and DLD1 (wt and shHsp70) and CM from these cells after incubation with etoposide or oxaliplatin. (C) MTT assay data of A549 and DLD1 cells incubated in CM from wt or shHsp70 cells after etoposide or oxaliplatin treatment for 14 days. (D) Data of xCelligence experiment on A549 and DLD1 cell growth in presence of Eto-CM or Oxa-CM from wt and shHsp70 cells. (E) Colony formation of A549 and DLD1 cells in presence of Eto-CM or Oxa-CM from wt and shHsp70 cells. (F) Colonies intensity, presented in folds of control. (G) Repopulation marker genes expression in A549 and DLD1 cells in presence of Eto-CM or Oxa-CM from wt and shHsp70 cells

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