Fig. 6
From: Autocrine regulation of tumor cell repopulation by Hsp70-HMGB1 alarmin complex
The Hsp70-HMGB1 complex is form inside cells and then releases to extracellular milieu. (A) Scheme of Protein-Protein Interaction assay (PPI). (B,C) A549 cell were treated with etoposide for 2, 4 and 8 h and then cell lysates (CL) (B) and cell media (CM) (C) were collected and amount of Hsp70-HMGB1 complexes was evaluated with PPI assay (as in scheme). (D) Data of confocal microscopy data for A549 cells treated with etoposide for 2 and 4 h and stained with antibodies against HMGB1 (green) and against Hsp70 (red). Nuclei were stained with DAPI. Scale bars: 10 μm