Fig. 7
From: Autocrine regulation of tumor cell repopulation by Hsp70-HMGB1 alarmin complex
Hsp70 inhibitors reduce Hsp70-HMGB1 complexes formation in CM and suppress tumor cells growth in vitro and in vivo.(A) PPI assay of purified Hsp70 and HMGB1 in presence of known Hsp70 inhibitors. (B) The number of Hsp70-HMGB1 complexes in A549 cell media after treatment with etoposide in presence of JG-98 or PES. * p < 0.05; ** p < 0.001. (C) Proliferation of A549 cells incubated in CM of A549 cells treated with etoposide alone or in presence of JG98 (upper panel) or PES (lower panel) estimated with the use of xCELLigence in real time. (D) PGE2 production by A549 cells treated with Eto-CM and Eto + JG98-CM for 24 h (E) Autophagy level in A549 cells treated with CM from untreated cells, Eto-CM, Eto + JG98-CM and Eto + PES-CM. (F) A549 cells, untreated and incubated in Eto-CM or Eto + JG98-CM, were subcutaneously injected into Balb/c nude mice (n = 10 in each group) and 41 days later were monitored using the IVIS Spectrum imaging system. Representative bioluminescence images. (G) Luminescence of tumor lesions. * p < 0.05, ** p < 0.005