Fig. 2

lnc-MLETA1 promotes migration and invasion of lung cancer cells in vitro. A RNA-sequencing of total RNA extracted from CL1-0 exosomes and CL1-5 exosomes are presented in a heatmap. B and C qRT-PCR analysis of lnc-MLETA1 and other candidate lncRNA expression in CL1-0 and CL1-5 exosomes (B) and cells (C). D qRT-PCR analysis of lnc-MLETA1 expression between low metastatic cells and high metastatic lung cancer cells. E Left: representative images of wound-healing assay of lnc-MLETA1-overexpressing and control CL1-0 cells for 16 h. Scale bar, 200 μm. Right: the number of migrated cells was counted. F Left: representative images of transwell invasion assay of lnc-MLETA1-overexpressing and control CL1-0 cells for 24 h. Scale bar, 200 μm. Right: the number of invaded cells was counted. G Left: representative images of wound-healing assay of lnc-MLETA1-knockdown and control CL1-5 cells for 10 h. Scale bar, 200 μm. Right: the number of migrated cells was counted. H Left: representative images of transwell invasion assay of lnc-MLETA1-knockdown and control CL1-5 cells for 16 h. Scale bar, 200 μm. Right: the number of invaded cells was counted I The XY position plots of single-cell tracking analysis of lnc-MLETA1-knockdown and control CL1-5 cells. J The path length and line length of cell migration of lnc-MLETA1-knockdown and control CL1-5 cells. Results are presented as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Two-tailed Student’s t-test