Fig. 3

Exosomal lnc-MLETA1 promotes lung cancer cell migration and invasion in vitro. A Immunofluorescence assay of biotin-labelled lnc-MLETA1 in CL1-0 cells pre-incubated with exosomes derived from biotin-labelled lnc-MLETA1-transfected cells or control CL1-0 cells. Cells were stained with rhodamine phalloidin and DAPI for F-actin and nuclei, respectively. White arrowheads indicate the biotin-labelled lnc-MLETA1. Scale bar, 20 μm. B Left: representative images of wound-healing assay of CL1-0 cells pre-incubated with exosomes derived from lnc-MLETA1-overexpressing or control CL1-0 cells for 48 h. Scale bar, 200 μm. Right: the number of migrated cells was counted. C Left: representative images of transwell invasion assay of CL1-0 cells pre-incubated with exosomes derived from lnc-MLETA1-overexpressing or control CL1-0 cells for 48 h. Scale bar, 200 μm. Right: the number of invaded cells was counted. D Left: representative images of wound-healing assay of CL1-0 cells pre-incubated with CL1-5 exosomes and transfected with lnc-MLETA1 LNA or control LNA for 48 h. Scale bar, 200 μm. Right: the number of migrated cells was counted. E Left: representative images of transwell invasion assay of CL1-0 cells pre-incubated with CL1-5 exosomes and transfected with lnc-MLETA1 LNA or control LNA for 48 h. Scale bar, 200 μm. Right: the number of invaded cells was counted. Results are presented as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01. Two-tailed Student’s t-test