Fig. 5

lnc-MLETA1 interacts with miR-186-5p and miR-497-5p to promote cell motility. A Cellular RNA fractionation assay of lnc-MLETA1 in CL1-5 cells. B Western blot analysis of Ago2 and MS2-GST expression in lysate and the MS2-TRAP RNA pull-down sample. C Schematic of predicted binding sites of miR-186-5p, miR-497-5p, miR-127-5p and miR-375 on lnc-MLETA1. D qRT-PCR analysis of lnc-MLETA1 expression in the MS2-TRAP RNA pull-down sample. E qRT-PCR analysis of miR-186-5p, miR-497-5p, miR-127-5p and miR-375 expression in the MS2-TRAP RNA pull-down sample. F Left: representative images of wound-healing assay of CL1-0 cells co-transfected with lnc-MLETA1 plasmids or control plasmids and with miRNA mimics or negative control (NC). Scale bar, 200 μm. Right: the number of migrated cells was counted. G Left: representative images of wound-healing assay of CL1-5 cells co-transfected with lnc-MLETA1 plasmids or control plasmids and with miRNA mimics or negative control. Scale bar, 200 μm. Right: the number of migrated cells was counted. Results are presented as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Two-tailed Student’s t-test